Journal: Oncology Letters

Article Title: Immunohistochemical profile and prognostic significance in primary central nervous system lymphoma: Analysis of 89 cases

doi: 10.3892/ol.2017.6893

Figure Lengend Snippet: Immunohistochemical labeling. (A and B) H&E staining. In tumor cells with the diffuse distribution, the size of nuclei were 2 times greater than of normal lymphocytes. (A) Magnification, ×200. (B) Magnification, ×400. (C) CD20 cell membrane staining performed using the EnVision method. Magnification, ×200. (D) CD10 cell membrane staining performed using the EnVision method. Magnification, ×200. (E) BCL-6, nuclei staining, EnVision method, ×100. (F) MUM-1 nuclei staining performed using the EnVision method. Magnification, ×200. (G) BCL-2 cytoplasmic staining performed using the EnVision method. Magnification, ×200. (H) Ki-67 nuclei staining performed using the EnVision method. Magnification, ×200. H&E, hematoxylin and eosin; CD, cluster of differentiation; BCL, B cell lymphoma; MUM-1, multiple myeloma-1.

Article Snippet: Monoclonal antibodies against CD20 (UM800002, 1:100 dilution), CD10 (UM870127, 1:600 dilution), BCL-6 (TA804186, 1:150 dilution), MUM1 (TA327705; 1:100-1:500 dilution), BCL-2 (UM870117, 1:500 dilution), Ki-67 (TA352729, 1:100), CD138 (TA327619, 1:25-1:200) were used and all purchased from OriGene Technologies, Inc. (Rockville, MD, USA).

Techniques: Immunohistochemical staining, Labeling, Staining

Journal: Medicina

Article Title: Repositioning Canagliflozin for Mitigation of Aluminium Chloride-Induced Alzheimer’s Disease: Involvement of TXNIP/NLRP3 Inflammasome Axis, Mitochondrial Dysfunction, and SIRT1/HMGB1 Signalling

doi: 10.3390/medicina60111805

Figure Lengend Snippet: Effect of the different doses of canagliflozin on the redox status and Nrf2 content of the hippocampal tissues (mean ± SD); * = p < 0.05, ** = p < 0.01, *** = p < 0.001. AlCl 3 , aluminium chloride; CNG, canagliflozin.

Article Snippet: The hippocampal tissue specimens were immunostained using an anti-BCL-2 monoclonal antibody (Boster Biological Technology, Pleasanton, CA, USA, catalogue number M00040-3).

Techniques:

Journal: Medicina

Article Title: Repositioning Canagliflozin for Mitigation of Aluminium Chloride-Induced Alzheimer’s Disease: Involvement of TXNIP/NLRP3 Inflammasome Axis, Mitochondrial Dysfunction, and SIRT1/HMGB1 Signalling

doi: 10.3390/medicina60111805

Figure Lengend Snippet: Effect of the different doses of canagliflozin on the hippocampal TXNIP/NF-κB p65/NLRP3 inflammasome signalling in rats injected with aluminium chloride (mean ± SD); *** = p < 0.001. AlCl 3 , aluminium chloride; CNG, canagliflozin.

Article Snippet: The hippocampal tissue specimens were immunostained using an anti-BCL-2 monoclonal antibody (Boster Biological Technology, Pleasanton, CA, USA, catalogue number M00040-3).

Techniques: Injection

Journal: Medicina

Article Title: Repositioning Canagliflozin for Mitigation of Aluminium Chloride-Induced Alzheimer’s Disease: Involvement of TXNIP/NLRP3 Inflammasome Axis, Mitochondrial Dysfunction, and SIRT1/HMGB1 Signalling

doi: 10.3390/medicina60111805

Figure Lengend Snippet: Effect of the different doses of canagliflozin on the hippocampal tissue levels of the pro-inflammatory cytokines in rats injected with aluminium chloride (mean ± SD); *** = p < 0.001. AlCl 3 , aluminium chloride; CNG, canagliflozin.

Article Snippet: The hippocampal tissue specimens were immunostained using an anti-BCL-2 monoclonal antibody (Boster Biological Technology, Pleasanton, CA, USA, catalogue number M00040-3).

Techniques: Injection

Journal: Medicina

Article Title: Repositioning Canagliflozin for Mitigation of Aluminium Chloride-Induced Alzheimer’s Disease: Involvement of TXNIP/NLRP3 Inflammasome Axis, Mitochondrial Dysfunction, and SIRT1/HMGB1 Signalling

doi: 10.3390/medicina60111805

Figure Lengend Snippet: Effect of the different doses of canagliflozin on SIRT1/HMGB1 signalling in the hippocampal tissue specimens of rats injected with aluminium chloride (mean ± SD); *** = p < 0.001. AlCl 3 , aluminium chloride; CNG, canagliflozin.

Article Snippet: The hippocampal tissue specimens were immunostained using an anti-BCL-2 monoclonal antibody (Boster Biological Technology, Pleasanton, CA, USA, catalogue number M00040-3).

Techniques: Injection

Journal: Medicina

Article Title: Repositioning Canagliflozin for Mitigation of Aluminium Chloride-Induced Alzheimer’s Disease: Involvement of TXNIP/NLRP3 Inflammasome Axis, Mitochondrial Dysfunction, and SIRT1/HMGB1 Signalling

doi: 10.3390/medicina60111805

Figure Lengend Snippet: Effect of the different doses of canagliflozin on the autophagy markers in the hippocampal tissue specimens of rats injected with aluminium chloride (mean ± SD); ns = non-significant, ** = p < 0.01, *** = p < 0.001. AlCl 3 , aluminium chloride; CNG, canagliflozin.

Article Snippet: The hippocampal tissue specimens were immunostained using an anti-BCL-2 monoclonal antibody (Boster Biological Technology, Pleasanton, CA, USA, catalogue number M00040-3).

Techniques: Injection

Journal: Medicina

Article Title: Repositioning Canagliflozin for Mitigation of Aluminium Chloride-Induced Alzheimer’s Disease: Involvement of TXNIP/NLRP3 Inflammasome Axis, Mitochondrial Dysfunction, and SIRT1/HMGB1 Signalling

doi: 10.3390/medicina60111805

Figure Lengend Snippet: Effect of the different doses of canagliflozin on caspase 3 and Bax levels in the hippocampal tissue specimens of rats injected with aluminium chloride (mean ± SD); *** = p < 0.001. AlCl 3 , aluminium chloride; CNG, canagliflozin.

Article Snippet: The hippocampal tissue specimens were immunostained using an anti-BCL-2 monoclonal antibody (Boster Biological Technology, Pleasanton, CA, USA, catalogue number M00040-3).

Techniques: Injection

Journal: Medicina

Article Title: Repositioning Canagliflozin for Mitigation of Aluminium Chloride-Induced Alzheimer’s Disease: Involvement of TXNIP/NLRP3 Inflammasome Axis, Mitochondrial Dysfunction, and SIRT1/HMGB1 Signalling

doi: 10.3390/medicina60111805

Figure Lengend Snippet: Effect of the different doses of canagliflozin on the mitochondrial ATP levels, mitochondrial complex I activity, and mitochondrial transmembrane potential in the hippocampal tissue specimens of rats injected with aluminium chloride (mean ± SD); * = p < 0.05, ** = p < 0.01, *** = p < 0.001. AlCl 3 , aluminium chloride; CNG, canagliflozin.

Article Snippet: The hippocampal tissue specimens were immunostained using an anti-BCL-2 monoclonal antibody (Boster Biological Technology, Pleasanton, CA, USA, catalogue number M00040-3).

Techniques: Activity Assay, Injection

Journal: Medicina

Article Title: Repositioning Canagliflozin for Mitigation of Aluminium Chloride-Induced Alzheimer’s Disease: Involvement of TXNIP/NLRP3 Inflammasome Axis, Mitochondrial Dysfunction, and SIRT1/HMGB1 Signalling

doi: 10.3390/medicina60111805

Figure Lengend Snippet: Photomicrographs representing the effect of the different treatments on the immune expression of BCL-2 in the hippocampal tissue specimens from the CA1 region of the studied groups. There is a significant decrease in the cytoplasmic immuno-expression of BCL-2 in the pyramidal cells in group II (aluminium chloride group). Groups III and IV treated with 5 mg/kg/day and 10 mg/kg/day canagliflozin, respectively, show moderate immuno-expression of BCL-2. In both groups I (control) and V (treated with 20 mg/kg/day canagliflozin), there is strong immunoreaction (BCL-2 immunostaining, ×40). *** = p < 0.001. AlCl 3 , aluminium chloride; CNG, canagliflozin.

Article Snippet: The hippocampal tissue specimens were immunostained using an anti-BCL-2 monoclonal antibody (Boster Biological Technology, Pleasanton, CA, USA, catalogue number M00040-3).

Techniques: Expressing, Control, Immunostaining

Journal: Medicina

Article Title: Repositioning Canagliflozin for Mitigation of Aluminium Chloride-Induced Alzheimer’s Disease: Involvement of TXNIP/NLRP3 Inflammasome Axis, Mitochondrial Dysfunction, and SIRT1/HMGB1 Signalling

doi: 10.3390/medicina60111805

Figure Lengend Snippet: Photomicrographs representing the effect of the different treatments on the immuno-expression of BCL-2 in the hippocampal tissue specimens from the CA3 region of the studied groups. There is a significant decrease in the cytoplasmic immuno-expression of BCL-2 in the pyramidal cells in group II (aluminium chloride group). Groups III and IV treated with 5 mg/kg/day and 10 mg/kg/day canagliflozin, respectively, show moderate immuno-expression of BCL-2. In both groups I (control) and V (treated with 20 mg/kg/day canagliflozin), there is strong immunoreaction (BCL-2 immunostaining, ×40). *** = p < 0.001. AlCl 3 , aluminium chloride; CNG, canagliflozin.

Article Snippet: The hippocampal tissue specimens were immunostained using an anti-BCL-2 monoclonal antibody (Boster Biological Technology, Pleasanton, CA, USA, catalogue number M00040-3).

Techniques: Expressing, Control, Immunostaining

Journal: Medicina

Article Title: Repositioning Canagliflozin for Mitigation of Aluminium Chloride-Induced Alzheimer’s Disease: Involvement of TXNIP/NLRP3 Inflammasome Axis, Mitochondrial Dysfunction, and SIRT1/HMGB1 Signalling

doi: 10.3390/medicina60111805

Figure Lengend Snippet: Photomicrographs representing the effect of the different treatments on the immuno-expression of BCL-2 in the hippocampal tissue specimens from the DG region of the studied groups. There is a significant decrease in the cytoplasmic immuno-expression of BCL-2 in the granular cells in group II (aluminium chloride group). Groups III and IV treated with 5 mg/kg/day and 10 mg/kg/day canagliflozin, respectively, show moderate immuno-expression of BCL-2. In both groups I (control) and V (treated with 20 mg/kg/day canagliflozin), there is strong immunoreaction (BCL-2 immunostaining, ×40). *** = p < 0.001. AlCl 3 , aluminium chloride; CNG, canagliflozin.

Article Snippet: The hippocampal tissue specimens were immunostained using an anti-BCL-2 monoclonal antibody (Boster Biological Technology, Pleasanton, CA, USA, catalogue number M00040-3).

Techniques: Expressing, Control, Immunostaining

Journal: Medicina

Article Title: Repositioning Canagliflozin for Mitigation of Aluminium Chloride-Induced Alzheimer’s Disease: Involvement of TXNIP/NLRP3 Inflammasome Axis, Mitochondrial Dysfunction, and SIRT1/HMGB1 Signalling

doi: 10.3390/medicina60111805

Figure Lengend Snippet: An illustrative diagram of the mechanisms by which the different doses of canagliflozin may mitigate the pathologic changes in the hippocampal tissues created by aluminium chloride injection.

Article Snippet: The hippocampal tissue specimens were immunostained using an anti-BCL-2 monoclonal antibody (Boster Biological Technology, Pleasanton, CA, USA, catalogue number M00040-3).

Techniques: Injection

Journal: International Journal of Molecular Medicine

Article Title: Semaglutide attenuates seizure severity and ameliorates cognitive dysfunction by blocking the NLR family pyrin domain containing 3 inflammasome in pentylenetetrazole-kindled mice

doi: 10.3892/ijmm.2021.5052

Figure Lengend Snippet: Immunofluorescence and WB analyses of apoptosis-related proteins in mouse hippocampal tissue. (A) Representative immunofluorescence images and (B) densitometric analysis of active caspase-3; both 10 and 25 nM/kg semaglutide decreased the fluorescence intensity of active caspase-3 in the CA1 and CA3 regions (one-way ANOVA). (C) Representative immunofluorescence images and (D) densitometric analysis of Bax showing that both doses of semaglutide decreased the fluorescence intensity of Bax in the CA1 and CA3 regions (one-way ANOVA). (E) Representative immunofluorescence images and (F) densitometric analysis of Bcl-2 showing that both 10 and 25 nM/kg semaglutide increased the fluorescence intensity of Bcl-2 in the CA1 and CA3 regions (one-way ANOVA). (G) Representative WB images of active caspase-3, Bax and Bcl-2 in the different groups. (H and I) Statistical results of the immunoblot analysis showing that 10 and 25 nM/kg semaglutide reduced the band intensity of active caspase-3 (one-way ANOVA) and increased the Bcl-2/Bax ratio (two-way ANOVA). Data are presented as the means ± SD. * P<0.05, ** P<0.01 and *** P<0.001. N≥3. WB, western blot/blotting; PTZ, pentylenetetrazole.

Article Snippet: To block non-specific binding, 4% bovine serum albumin (BSA) was applied at room temperature (RT) for 1.5 h, after which the membrane was incubated with the following primary antibodies for 17 h at 4°C: Anti-NLRP3 (cat. no. bs-10021R), anti-ASC (cat. no. bs-6741R), anti-IL-1β, (cat. no. bs-0812R) and anti-caspase-1p20 (cat. no. bs-10442R) (all 1:500); anti-IL-6, (cat. no. bs-6309R), anti-IL-18 (cat. no. bs-4988R), anti-TNF-α (cat. no. bs-10802R), anti-active caspase-3 (cat. no. bsm-33199M), anti-Bax (cat. no. bs-0127R), anti-Bcl-2 (cat. no. bsm-52304R) and anti-β-actin (cat. no. bs-0061R) (all 1:1,000) (BIOSS); and anti-β-actin (cat. no. 66009-1-Ig; 1:5,000; ProteinTech Group, Inc.).

Techniques: Immunofluorescence, Fluorescence, Western Blot

Journal: Cancer Communications

Article Title: Phosphatidylserine released from apoptotic cells in tumor induces M2‐like macrophage polarization through the PSR‐STAT3‐JMJD3 axis

doi: 10.1002/cac2.12272

Figure Lengend Snippet: Apoptotic tumor cells correlated with M2‐like macrophages in tumor. (A) Representative immunofluorescence images of apoptotic tumor cells (Cleaved Caspase‐3 + , green) and macrophages (CD163 + , red) in human colon cancer specimens are shown (left: more apoptotic tumor cells; right: fewer apoptotic cells). (B‐D) Correlation analyses for the number of apoptotic tumor cells and macrophages in human colon cancer (B), breast cancer (C), and lung cancer (D) are shown. (E) Representative immunofluorescence images of apoptotic cells (Cleaved Caspase‐3 + , green) and macrophages (F4/80 + , red) in mouse CT26 colon cancer specimens are shown (left: more apoptotic tumor cells; right: fewer apoptotic cells). (F‐H) Correlation analyses for the number of apoptotic tumor cells and macrophages in CT26 (F), 4T1 (G), or LL2 (H) subcutaneous tumor are shown the number of apoptotic tumor cells or macrophages per specimen were calculated by the mean number counted in ten HPFs. The Pearson correlation coefficieI(r) and significance levels ( P ) are presented. (I) 72 h after peritoneal injection of different CT26 cells (Control, Bcl2, or shBcl2), the accumulation of M2 macrophages (CD45 + CD11b + F4/80 + CD206 + ) in the peritoneal cavity were detected by flow cytometry. (J) Infiltration of M2 type macrophages (CD11b + F4/80 + CD206 + ) in CT26 malignant ascites with or without DNR treatment (1 mg/kg, once) are shown. (K) Chemotherapy‐induced apoptotic CT26 cells (DNR‐CT26) elevated CD206 + macrophage accumulation in peritoneal cavity. Data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01, and *** P < 0.001 calculated using a two‐tailed unpaired Student's t‐test Abbreviations: HPF, high power field; DNR, daunorubicin.

Article Snippet: The murine Bcl2 gene (GenBank accession number NM_009741) was cloned by OriGene Technologies Inc (Rockville, MD, USA).

Techniques: Immunofluorescence, Injection, Flow Cytometry, Two Tailed Test